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human hepatoma hep3b cells  (ATCC)


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    ATCC human hepatoma hep3b cells
    Human Hepatoma Hep3b Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human hepatoma hep3b cells/product/ATCC
    Average 98 stars, based on 1199 article reviews
    human hepatoma hep3b cells - by Bioz Stars, 2026-04
    98/100 stars

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    human hepatoma hep3b cells  (ATCC)
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    Human Hepatoma Hep3b Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mt2 expression markedly reduces Neo1 levels in <t>Hep3B</t> cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.
    Hep3b Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell culture conditions hep3b  (ATCC)
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    Mt2 expression markedly reduces Neo1 levels in <t>Hep3B</t> cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.
    Cell Culture Conditions Hep3b, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mt2 expression markedly reduces Neo1 levels in <t>Hep3B</t> cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.
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    mammalian cell culture hep3b  (ATCC)
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    Mt2 expression markedly reduces Neo1 levels in <t>Hep3B</t> cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.
    Mammalian Cell Culture Hep3b, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human hcc cell lines hep3b  (ATCC)
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    ATCC human hcc cell lines hep3b
    Mt2 expression markedly reduces Neo1 levels in <t>Hep3B</t> cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.
    Human Hcc Cell Lines Hep3b, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    transfections hep3b cells  (ATCC)
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    Mt2 expression markedly reduces Neo1 levels in <t>Hep3B</t> cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.
    Transfections Hep3b Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human hcc cell lines hep3b hb 8064  (ATCC)
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    ATCC human hcc cell lines hep3b hb 8064

    Human Hcc Cell Lines Hep3b Hb 8064, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Mt2 expression markedly reduces Neo1 levels in Hep3B cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.

    Journal: The Journal of Biological Chemistry

    Article Title: Matriptase-2-mediated suppression of hepatic hepcidin expression in mice requires hepatocyte neogenin

    doi: 10.1016/j.jbc.2026.111142

    Figure Lengend Snippet: Mt2 expression markedly reduces Neo1 levels in Hep3B cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.

    Article Snippet: We obtained Hep3B cells from ATCC (#HB-8064).

    Techniques: Expressing

    Journal: bioRxiv

    Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

    doi: 10.1101/2025.11.27.691042

    Figure Lengend Snippet:

    Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

    Techniques: Concentration Assay

    Modulation of paraxanthine accumulation by known CYP1A2 effectors in HepG2 and Hep3B cells. Paraxanthine concentration was quantified by LC–MRM. Data are shown as mean ± SD from three independent experiments. A-B, Sulforaphane reduced paraxanthine accumulation in HepG2 and Hep3B cells. C-D , Galangin increased paraxanthine accumulation in both cell lines. E-F , 3-Methylcholanthrene (3-MC) also increased paraxanthine accumulation in both cell lines. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: bioRxiv

    Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

    doi: 10.1101/2025.11.27.691042

    Figure Lengend Snippet: Modulation of paraxanthine accumulation by known CYP1A2 effectors in HepG2 and Hep3B cells. Paraxanthine concentration was quantified by LC–MRM. Data are shown as mean ± SD from three independent experiments. A-B, Sulforaphane reduced paraxanthine accumulation in HepG2 and Hep3B cells. C-D , Galangin increased paraxanthine accumulation in both cell lines. E-F , 3-Methylcholanthrene (3-MC) also increased paraxanthine accumulation in both cell lines. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

    Techniques: Concentration Assay

    qPCR measurement of CYP1A2 mRNA levels in HepG2 (top panels) and Hep3B (bottom panels) cells after 24-hour treatments with A-B , caffeine and caffeine along with C-D, D,L-sulforaphane, E-F , 3-methylcholanthrene, or G-H , galangin at the indicated concentrations. Control cells received the vehicle only. Data are normalized to a housekeeping gene and expressed as the mean ± SD (n = 3) from three independent experiments. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: bioRxiv

    Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

    doi: 10.1101/2025.11.27.691042

    Figure Lengend Snippet: qPCR measurement of CYP1A2 mRNA levels in HepG2 (top panels) and Hep3B (bottom panels) cells after 24-hour treatments with A-B , caffeine and caffeine along with C-D, D,L-sulforaphane, E-F , 3-methylcholanthrene, or G-H , galangin at the indicated concentrations. Control cells received the vehicle only. Data are normalized to a housekeeping gene and expressed as the mean ± SD (n = 3) from three independent experiments. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

    Techniques: Control